1. The study focuses on optimizing antimicrobial nanocomposite films using carboxymethyl cellulose, Commiphora mukul polysaccharide, and Chitosan Nanofiber. 2. Various concentrations of these components were tested to enhance film properties such as strength, moisture content, and antimicrobial activity. 3. The optimized formulation showed improved physical, mechanical, and antibacterial properties, suitable for applications in food packaging and biomedical fields.